电离辐射通过转化生长因子-β-介导的上皮-间质转换来促进细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

概述 ：探讨伽马射线是否可通过转化介素-β（TGF-β）-依赖性的上皮-游离转换 (EMT)来促进骨髓的侵袭迁移。适用增幅2Gy(60)Coγ线反射源自本能生殖器官的6种骨髓，记录与EMT相关的变化，这仅限于分别借助于显微镜技术，蛋白内印迹作法律条文，免疫荧光技术，样试验和Transwell小室试验来观察并监测蛋白组织型态，EMT上面，侵袭迁移战斗能力等。采用核糖体联免疫吸附法律条文监测这些骨髓中都TGF-β复合物准确度，借助于特别减缓剂SB431542来评估TGF-β波形闭环在伽马射线EMT中都的起着。经过增幅为2Gy反射的骨髓中都存在间叶蛋白的表达，与；也反射组相比其上皮上面减少，间叶蛋白上面增加，同时其侵袭转回战斗能力弱化，TGF-β复合物准确度也提高。进一步发掘出由A549伽马射线诱导的EMT可通过对TGF-β波形减缓时有发生逆转。这些结果表明TGF-β依赖性的EMT在伽马射线诱导弱化骨髓侵袭转回战斗能力中都起着关键起着。

编辑： lizexiu